RBS

Part:BBa_K5282010:Design

Designed by: Chengyao Yin   Group: iGEM24_HS-Beta   (2024-10-01)


TfCa CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
    Illegal NotI site found at 1498
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal BamHI site found at 308
    Illegal XhoI site found at 1507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 74
    Illegal EcoRI site found at 163
    Illegal EcoRI site found at 1195
    Illegal PstI site found at 803
    Illegal NgoMIV site found at 826
    Illegal NgoMIV site found at 904
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1327
    Illegal SapI.rc site found at 1315


Design Notes

Fusion expression of His tag was used for purification in vitro.


Source

It comes from the genomic sequence of Thermobifida fusca.

References